SunRail operates a bus from Sanford to Gainesville every 30 minutes. Tickets cost $2 - $4 and the journey takes 42 min. 2 other operators also service this route.
How to get from Sanford to Gainesville
From Sanford train to Lynx Central Station - Sunrail, line 104 bus to Orlando, FL, then Megabus to Gainesville, FL. 4 h 12 min $19. From Sanford train to Sand Lake Road, walk to Orlando - RedCoach Station, FL, then bus to Gainesville. 4 h 38 min $43. From Sanford drive to Gainesville. 2 h 14 min $13.