Greyhound USA operates a bus from Orlando to Valdosta once daily. Tickets cost $26 - $65 and the journey takes 4 h 48 min. Alternatively, Delta, Southwest Airlines and 2 other airlines fly from Orlando to Valdosta every 2 hours.
From Orlando, FL bus to Valdosta, GA. 4 h 48 min $49. From Orlando drive to Valdosta. 3 h 30 min $25. From Church Street train to Sand Lake Rd Sunrail Station, line 111 bus to Orlando airport, fly to Valdosta airport, then taxi to Valdosta. 5 h 36 min $493.