Reina del Camino operates a bus from Guayaquil to Portoviejo hourly. Tickets cost $6 and the journey takes 4 h. Cooperativa Coactur Manabí also services this route every 4 hours.
How to get from Guayaquil to Portoviejo
From Guayaquil taxi to Guayaquil Terminal, Avenida Benjamín Rosales, La Garzota, Tarqui, Guayaquil, Guayas, Ecuador then bus to Portoviejo. 4 h 10 min $23. From Guayaquil drive to Portoviejo. 2 h 57 min.