Trenitalia Intercity operates a train from Ancona to Assisi every 3 hours. Tickets cost $20 - $30 and the journey takes 1 h 42 min. 2 other operators also service this route. Alternatively, Contram Mobilita s.c.p.a. operates a bus from Ancona to Assisi every 2 hours, and the journey takes 1 h 24 min. 2 other operators also service this route.
How to get from Ancona to Assisi
From Ancona train to Foligno, train to Assisi Stazione, then bus to P.za Matteotti Assisi. 3 h 4 min 28€. From Ancona P.Zza Ugo Bassi bus to Macerata Autostazione, bus to V.L.Angeles S.M.D.Angeli, then bus to P.za Matteotti Assisi. 4 h 35 min 21€. From Ancona drive to Assisi. 1 h 44 min 18€.