The average bus between Mainz and Jena takes 6h 20m and the fastest bus takes 5h. The bus service runs several times per day from Mainz to Jena. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Buses run twice daily between Mainz and Jena. The earliest departure is at 5:10 AM in the morning, and the last departure from Mainz is at 10:15 PM which arrives into Jena at 5:35 AM. All services require a transfer, and take on average 6h 20m. The schedules shown below are for the next available departures.

7h 55m • 1 change

5h • 1 change
Arrives next day

7h 20m • 1 change

7h 55m • 1 change

5h • 1 change
Arrives next day

7h 20m • 1 change
Want to know about travelling from Mainz to Jena? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct bus from Mainz to Jena. However, there are services departing from Mainz station and arriving at Jena station via Jena bus station station. The journey, including transfers, takes approximately 6h 20m.
Mainz to Jena bus services, operated by FlixBus, depart from Frankfurt central train station.
Mainz to Jena bus services, operated by FlixBus, arrive at Jena bus station.
Book your Mainz to Jena bus tickets online with FlixBus.
The distance between Mainz and Jena is 256.8 km. The road distance is 331 km.
FlixBus operates a bus from Frankfurt central train station to Jena bus station 4 times a day. Tickets cost €21–35 and the journey takes 3h 35m.