The average bus between Brno and Jena takes 7h 58m and the fastest bus takes 7h 35m. The bus service runs several times per day from Brno to Jena. The journey time may be longer on weekends and holidays; use the search form on this page to search for a specific travel date.
Buses run 3 times a day between Brno and Jena. The earliest departure is at 5:30 AM in the morning, and the last departure from Brno is at 7:10 PM which arrives into Jena at 2:45 AM. All services require a transfer, and take on average 7h 58m. The schedules shown below are for the next available departures.

8h 10m • 1 change

8h 10m • 1 change
Arrives next day

7h 35m • 1 change

8h 10m • 1 change

8h 10m • 1 change
Arrives next day

7h 35m • 1 change

8h 10m • 1 change

8h 10m • 1 change
Arrives next day

7h 35m • 1 change

8h 10m • 1 change

8h 10m • 1 change
Arrives next day

7h 35m • 1 change

8h 10m • 1 change

8h 10m • 1 change
Arrives next day

7h 35m • 1 change
Want to know about travelling from Brno to Jena? We have put together a list of the most frequently asked questions from our users such as: What is the cheapest mode of transport? What is the quickest option? How much do tickets usually cost? and many more.
No, there is no direct bus from Brno to Jena. However, there are services departing from Brno station and arriving at Jena station via Praha, ÚAN Florenc. The journey, including transfers, takes approximately 7h 58m.
Brno to Jena bus services, operated by RegioJet, depart from Brno station.
Brno to Jena bus services, operated by RegioJet, arrive at Jena bus station.
Book your Brno to Jena bus tickets online with RegioJet and FlixBus.
The distance between Brno and Jena is 406.7 km. The road distance is 544 km.
You can take a bus from Brno to Jena bus station via Praha, ÚAN Florenc in around 8h 40m.